Product introduction
Summary
Staphylococcal aureus Enterotoxin Typing ELISA Test Kit adopts the double antibody sandwich ELISA method. Precoat the total amount of Staphylococcus aureus enterotoxin antibody on the microplate. Both the total amount of precoated enterotoxin antibody and detection antibody binds to the enterotoxin in the sample at the same time. And then the complex combines with the HRP-conjugate to form an antibody enterotoxin-antibody-enzyme-HRP-conjugate, which can be colored by TMB substrate. The absorbance of the sample is positively correlated with the content of Staphylococcus aureus enterotoxin.
Intended Use?
This test kit can qualitatively detect the content of Staphylococcus aureus enterotoxin A, B, C, D, and E in raw milk, finished milk, and fermented milk sample.
Ordering Information
| Catalog No.? | Sensitivity | Specification |
| LR50511 | 0.025-1 ppb | 96T |
Customized products available. Please consult you sales representative for detailed information
Precautions
1. If the kit or sample is not returned to room temperature(20-25℃), the OD value will be lower than normal.
2. If the plate wells are dry during the washing process, the repeatability will be poor. Therefore, the next step should be carried out immediately after the plate is washed and patted dry.
3. Shake well before adding every reagent.
4. The stop solution is 2M sulfuric acid. Avoid contact with skin and mucous membranes. Immediately clean up any spills and wash the area with copious amounts of water.
5. Do not use reagents after the expiration date. Do not substitute reagents from any other manufacturer into the test kit. Do not combine reagents from other ELISA Kits with different Lot numbers.
6. The kit should be stored at a temperature of 2-8℃. Do not freeze the kit. Seal the unused plates into the original package and reseal them. The reagent is light-sensitive. Avoid exposure to light directly.
7. Signs of reagent deterioration. Any color in the reagent indicates that the reagent has deteriorated and should be discarded.
8. After adding substrate A and substrate B, generally the color will develop in 15 minutes. If the color is light, the reaction time can be extended to 20 minutes. On the contrary, the reaction time can be shortened.
9. When the concentrated washing solution and other reagent components crystallize, please heat (the temperature should not exceed 60 ℃) to dissolve before use.
More Products
